18 th Annual Meeting Society of Virology

th

18 Annual Meeting Society of Virology Heidelberg, Germany March 5-8, 2008

In cooperation with DVV e.V.

Hygiene-Institut Universitätsklinikum Heidelberg

Deutsches Krebsforschungszentrum DKFZ Heidelberg

Workshop: Clinical virology Chairs:

Astrid Meerbach (Jena) Jaques Rohayem (Dresden)

When & Where:

Wednesday, March 5th, 2008 16:00-17:45

Zool. - Großer Saal

1.

Molecular epidemiology of a large hantavirus outbreak in Germany, 2007 Jörg Hofmann, *Helga Meisel, Boris Klempa, Silvan M. Vesenbeckh, Robert Beck, Detlef Michel, Jonas Schmidt-Chanasit, Rainer Ulrich, Sebastian Grund, Gisela Enders, Detlev H. Krüger

p. 341

2.

New insights into BK virus phylogeny and evolution *Andi Krumbholz , Olaf Bininda-Emonds, Renate Egerer, Roland Zell

p. 342

3.

Characterization of the translation strategy of the human pathogenic sapovirus in mammalian cells *Julia Gebhardt, Dorothea Kramer, Jacques Rohayem

p. 343

4.

High-throughput expression of the Varicella-Zoster Virus (VZV) proteome for serum profiling experiments

p. 344

*Armin Baiker, Angelika Lueking, Maria Vizoso Pinto, Raveendra Pothineni, Ann Arvin, Gundula Jaeger 5.

Nosocomial hepatitis C: enigmatic transmissions from patient to patient *Bernd Kupfer, Anna Maria Eis-Hübinger, Uwe Pöge, Hans Ulrich Klehr, Gert Schilling, Luba Lendowski, Martin Exner, Steffen Engelhart, Ingvill Purr, Bertfried Matz

p. 345

6.

Frequent detection of HHV6-, EBV- and CMV-DNA in liver biopsies of liver transplant recipients *Ilka Engelmann, Sven Pischke, Juliane Gösling, Cornelia HenkeGendo, Albert Heim

p. 346

7.

Prevalence and mean number of cutaneous HPV types increase with age Marc Gottschling, Mandy Lehmann, Anja Köhler, Eggert Stockfleth, *Ingo Nindl

p. 347

*: Presenting author

Workshop: Transformation and oncogenesis Chairs:

Armin Ensser (Erlangen) Ingo Nindl (Berlin)

When & Where:

Wednesday, March 5th, 2008 16:00-17:45

CH – Kleiner Saal

1.

Strong induction of 4-1BB, a growth and survival promoting receptor, in HTLV-1-infected cultured and patients’ T cells by the viral Tax oncoprotein *Klemens Pichler, Tarek Kattan, Juliane Gentzsch, Andrea K Kress, Graham P Taylor, Charles M Bangham , Ralph Grassmann

p. 439

2.

A Role for the Kaposi’s Sarcoma Herpesvirus FLICE Inhibitory Protein (vFLIP) in establishment of latency and the Formation of Endothelial Spindle Cell *Khaled Alkharsah, Antje Bürger, Oliver Dittrich-Breiholz, Michael Kracht, Matthias Thurau, Michael Stürzl, Thomas Schulz

p. 441

3.

Pro- and anti-apoptotic factors induced by Herpesvirus saimiri and Herpesvirus ateles oncoproteins Jill de Jong , Stefan Richter, Anja Glanz, Kerstin Wanke, Stefanie Heinemann, Jens-Christian Albrecht, Brigitte Biesinger

p. 444

4.

Methylation of the Arginine-Glycine repeat of the Epstein-Barr Virus p. 438 Nuclear Antigen 2 (EBNA2) generates functionally different EBNA2 species *Alfredo Mamiani, Stephanie Barth, Henrik Groß, Jarek Szcyrba, Christine Hennard, Elisabeth Kremmer, Friedrich A. Grässer

5.

Cell cycle independent expression of ie3 leads to G1 and G2 arrest in MCMV infected cells *Lüder Wiebusch, Anke Neuwirth, Linus Grabenhenrich, Sebastian Voigt, Christian Hagemeier

6.

HBV replication induces beta-catenin-signalling by activation of Src p. 442 kinases Gesa von Olshausen, Maria Quasdorff, Carien Niessen, *Ulrike Protzer

7.

Adenovirus type 5 early region 1B 55-kDa oncoprotein promotes cell transformation by a mechanism independent from blocking p53-activated transcription Barbara Härtl, *Peter Wimmer, Kremmer Elisabeth, Thomas Dobner

*: Presenting author

p. 440

p. 443

Workshop: Adaptive immune response and vaccination I Chairs:

Gerd Sutter (Langen) Alexandra Trkola (Zürich)

When & Where:

Thursday, March 6th, 2008 11:00-12:45

CH – Großer Saal

1.

A New Influenza B Live Vaccine Generated by Modification of the Haemagglutinin Cleavage Site *Jürgen Stech, Holger Garn, Astrid Herwig, Bianca Dauber, Thorsten Wolff, Thomas C. Mettenleiter, Hans-Dieter Klenk

p. 261

2.

Protection of cats against lethal influenza H5N1 challenge infection. *Thomas W. Vahlenkamp, Timm C. Harder, Matthias Giese, Jens P. Teifke, Martin Beer, Thomas C. Mettenleiter

p. 266

3.

p. 272 Cellular and humoral immune responses correlate with vaccine protective efficacy against the 1918 (H1N1) influenza virus in ferrets *Veronika von Messling, Darwyn Kobasa, Stephane Pillet, Michael Gray, Jason Gren, Shane Jones, Betty Luy, Tracy Taylor, Dominique Laddy, David Weiner, Heinz Feldmann, Gary P Kobinger

4.

Protective immunity against a retrovirus by immunisation with its transmembrane envelope (TM) protein Stefan Langhammer, Janine Huebner, Oswald Jarrett, Reinhard Kurth, *Joachim Denner

p. 262

5.

Kinetics of CD8+ effector T cell responses and induced CD4+ regulatory T cell responses in different organs during acute retrovirus infection *Gennadiy Zelinskyy, Kirsten Esperstedt, Simone Schimmer, Tim Sparwasser, Ulf Dittmer

p. 264

6.

Antigenic Structure of Tick-Borne Encephalitis Virus and Impact of Quaternary Organization on the Binding of Neutralizing Monoclonal Antibodies *Stefan Kiermayr, Karin Stiasny, Franz X. Heinz

p. 265

7.

Impact of MHC class I-associated selection pressure on the evolution of HCV NS3 in a single-source outbreak *Joerg Timm, Manfred Wiese, Jacob Nattermann, Marina Klein, Ulrich Spengler, Sergei Viazov, Michael Roggendorf

p. 267

*: Presenting author

Workshop: Viral vectors and gene therapy Chairs:

Anja Ehrhardt (München) Dirk Grimm (Heidelberg)

When & Where:

Thursday, March 6th, 2008 11:00-12:45

Zool. – Großer Saal

1.

Evaluation of infectious laryngotracheitis virus recombinants as vectored live vaccines against H5N1 avian influenza virus infection of chickens *Sophia P. Pavlova, Jutta Veits, Thomas C. Mettenleiter, Walter Fuchs

p. 228

2.

Equine Herpesvirus 1 (EHV-1) - a Promising Tool Regarding Heterologous Viral Gene Delivery for Vaccination Purposes *Helga Hofmann-Sieber, Jens Wild, Katharina Boeckl, Karsten Tischer, Jens von Einem, Nikolaus Osterrieder, Josef Koestler, Ralf Wagner

p. 229

3.

Systemic transfer of delta-sarcoglycan with targeted AAV vectors prevents heart failure in delta-sarcoglycan knock-out mice Caroline Goehringer, Desiree Rutschow, Stefanie Schinkel, Ralf Bauer, Raffi Bekeredjian, Dieter Weichenhan, Hugo A. Katus, Jürgen A. Kleinschmidt, *Oliver J. Müller

p. 230

4.

Gene-deleted adenoviral vector genomes exist predominantly as linear monomers: Implications for persistence of recombinant adenoviral vectors *Lorenz Jäger, Anja Ehrhardt

p. 231

5.

Effective and flexible cell entry targeting of lentiviral vectors through pseudotyping with variant measles virus H and F proteins Sabrina Funke, Andrea Maisner, Manuel Grez, Roberto Cattaneo, Christian J. Buchholz, *Klaus Cichutek

p. 232

6.

Molecular analysis of AAV concatemers and proviruses *Ali Nowrouzi, Karim Zaoui, Anna Schneider, Anne Arens, Manfred Schmidt, Christof von Kalle

p. 233

7.

“Armed” Oncolytic Adenoviruses: Deletion of the E1B19K Gene Modulates Oncolytic Activity But Does Not Interfere with the Expression of Transgenes *Stanimira Rohmer, Andrea Hesse, Christina Quirin, Dirk M. Nettelbeck

p. 234

*: Presenting author

Workshop: Innovative diagnostic methods Chairs:

Maren Eggers (Stuttgart) Daniela Huzly (Freiburg)

When & Where:

Thursday, March 6th, 2008 11:00-12:45

CH – Kleiner Saal

1.

Recombinant expression of all Hepatitis E Virus (HEV) antigens for the development of novel diagnostic tools *Andreas Osterman, Rudolf Haase, Anja Ehrhardt, Hans Nitschko, Armin Baiker

p. 373

2.

Combination of native and recombinant cytomegalovirus antigens in a new ELISA for detection of CMV-specific antibodies *Clemens Busse, Andreas Strubel, Paul Schnitzler

p. 374

3.

Rapid in depth diagnostics of viral full length genomes using the Genome Sequencer technology *Dirk Höper, Bernd Hoffmann, Martin Beer

p. 375

4.

Detection of contaminations in cell lines by novel multiplex assay *Markus Schmitt, Michael Pawlita

p. 376

*: Presenting author

AND Workshop: Systems biology of viral infections Chairs:

Jürgen Haas (München) Maik Lehmann (Heidelberg)

When & Where:

Thursday, March 6th, 2008 11:00-12:45

CH – Kleiner Saal

1.

Essential impact of NF-kappaB signaling on the H5N1 influenza A virus mediated cytokine response *Mirco Schmolke, Dorothee Viemann, Johannes Roth, Stephan Ludwig

p. 495

2.

Analysis of Intraviral Protein-Protein Interactions of the SARS Coronavirus ORFeome by peptidescanning *Albrecht von Brunn, Claudia Ester, Julia Schöpf, Frank Ebel, Peter Uetz, Jürgen Haas

p. 496

3.

High-throughput siRNA screening to identify human host factors involved in pathogenesis of HIV-1 Maik Jörg Lehmann, *Johannes Hermle, Kathleen Börner, Lars Kaderali, Holger Erfle, Rainer Pepperkok, Hans-Georg Kräusslich

p. 497

Workshop: Viral DNA genome replication Chairs:

Wolfram Brune (Berlin) Nikolaus Osterrieder (Berlin)

When & Where:

Thursday, March 6th, 2008 16:30-18:45

Zool. – Großer Saal

1.

DHBV epsilon RNA mutants with drastically altered upper stem regions are still initiation and replication competent *Markus Gajer, Michael Nassal

p. 27

2.

A Four-Phase Model of Human Cytomegalovirus Chromatin Assembly and Disassembly During Lytic Infection *Alexandra Nitzsche, Christina Paulus, Michael Nevels

p. 28

*: Presenting author

AND Workshop: Transcription and translation Chairs:

Jens Bohne (Hannover) Frank Stubenrauch (Tübingen)

When & Where:

Thursday, March 6th, 2008 16:30-18:45

Zool. – Großer Saal

p. 104

1.

Epigenetic modification of Epstein-Barr virus DNA is pivotal for viral latency and reactivation of de novo virus synthesis *Markus Kalla, Dagmar Pich, Wolfgang Hammerschmidt

2.

Non-covalent interaction of the cellular protein sumo with the major p. 100 HCMV transactivator IE2p86 affects its transactivation capacity and sumoylation *Anja Berndt, Heike Hofmann, Thomas Stamminger

3.

Viral Oncogene Promoter and Cell-Specific Transcriptional Regulation by Papillomavirus E2 Proteins *Matthias Ottinger, Jennifer A Smith, Michal-Ruth Schweiger, Peter M Howley

p. 103

4.

Regulated read-through at the first transcription termination site determines viral protein levels in Borna disease virus infection *Marion Poenisch, Sandra Wille, Peter Staeheli, Urs Schneider

p. 99

5.

Murine leukemia virus evolved an exceptional strategy to ensure alternative splicing via dual attenuation of the 5’ splice site *Daniela Zychlinski, Steffen Erkelenz, Janine Kraunus, Melanie Galla, Nicole Fischer, Heiner Schaal, Christopher Baum, Jens Bohne

p. 102

6.

Re-enaction of HIV-1 alternative splicing regulation by simultaneous application of an enhanced MS2- and PP7-tethering system *Steffen Erkelenz, Sarah Rosin, Heiner Schaal

p. 105

7.

IRES elements – tools for attenuation of Mononegavirales translation and replication *Adriane Marschalek, Stefan Finke, Martin Schwemmle, Daniel Mayer, Bernd Heimrich, Lothar Stitz, Karl-Klaus Conzelmann

p. 101

*: Presenting author

Workshop: Innate immune response Chairs:

Alexander Weber (Heidelberg) Friedemann Weber (Freiburg)

When & Where:

Thursday, March 6th, 2008 16:30-18:45

CH – Großer Saal

1.

Defining Substrate Specificities of RIG-I and Mda5 *Marco Binder, Florian Eberle, Volker Lohmann, Alexander Dalpke, Ralf Bartenschlager

p. 151

2.

Hepatitis A Virus Protein 2B Suppresses IRF-3 Activation by Interfering with MAVS-Dependent Signaling Resulting in Inhibition of IFN-beta *Dajana Paulmann, Thomas Magulski, Rebecca Schwarz, Andreas Dotzauer, Angelika Vallbracht

p. 147

3.

Measles virus V protein is a decoy substrate for the kinase IKKalpha and prevents Toll-like receptor 7/9-mediated IFN induction *Christian Pfaller, Karl-Klaus Conzelmann

p. 150

4.

Interferon-antagonistic ML protein of Thogoto virus targets general transcription factor IIB *Carola Vogt, Ellen Preuß, Daniel Mayer, Martin Schwemmle, Friedemann Weber, Georg Kochs

p. 149

5.

Inhibition of specific cytokine secretion pathways in plasmacytoid dendritic cells (pDC) by PCV2 *Carole Balmelli, Nadja Peduto, Artur Summerfield, Kenneth McCullough

p. 153

6.

Inhibition of the IFN-mediated signaling by the M1 protein of influenza A Virus *Jovan Pavlovic (1), Bettina Oberle (1), Karin Moelling (1)

p. 152

7.

Neutralization of PKR by the influenza B virus NS1 protein: Evidence for a crucial NS1-PKR complex that is bridged by dsRNA Bianca Dauber, Jana Schneider, Zoe Waibler, Ulrich Kalinke, *Thorsten Wolff

p. 148

8.

Interferon-lambda Contributes to Influenza Virus Resistance *Markus Mordstein, Georg Kochs, Laure Dumoutier, Jean-Christophe Renauld, Soren R. Paludan, Kevin Klucher, Peter Staeheli

p. 146

9.

Human papillomavirus type 8 early proteins deregulate immune homeostasis in skin Tanya Sperling, Gian Paolo Marcuzzi, Claudia Wickenhauser, Herbert Pfister, Slawomir Majewski, *Sigrun Smola-Hess

p. 154

Workshop: Epidemiology and zoonoses Chairs:

Martin Beer (Insel Riems) Paul Becher (Gießen)

When & Where:

Thursday, March 6th, 2008 16:30-18:45

CH – Kleiner Saal p. 460

1.

PB1-F2 of German porcine and avian influenza A viruses *Andi Krumbholz, Annett Eitner, Reimar Krieg, Hartmut Oehring, KarlJürgen Halbhuber, Peter Wutzler, Roland Zell

2.

SARS-Coronavirus inhibits interferon induction both at pre- and the p. 461 post-transcriptional levels *Thomas Kuri, Xiaonan Zhang, Louis Martinez-Sobrido, Adolpho GarcíaSastre, Zhenghong Yuan, Friedemann Weber

3.

First Germany-wide epidemiology of hantavirus infections in rodent p. 462 reservoir hosts *Rainer G. Ulrich, Mathias Schlegel, Jonas Schmidt-Chanasit, Boris Klempa, Marc Mertens, Daniel Masur, Thomas Büchner, Kati Sevke, Jona Freise, Jens Jacob, Detlev H. Krüger, Rainer Oehme, Stefan O. Brockmann, Gerald Heckel, Sandra S. Essbauer

4.

Human Papillomavirus 16 is the predominant type etiologically involved in penile squamous cell carcinoma Daniëlle A.M. Heideman, *Tim Waterboer, Michael Pawlita, Pien Delisvan Diemen, Ingo Nindl, Joost A. Leijte, Johannes M.G. Bonfrer, Simon Horenblas, Chris J.L.M. Meijer, Peter J.F. Snijders

p. 463

5.

Evidence of Interspecies Transmission and Reassortment Among Avian Group A Rotaviruses Thomas Schumann, Helmut Hotzel, Peter Otto, *Reimar Johne

p. 464

6.

First isolation and genetic characterization of two tick-borne encephalitis viruses in Germany since 30 years *Sandra Essbauer, Gudrun Zöller, Astrid Thomas, Lothar Zöller, C Klaus, Jochen Suess, B Stark, Martin Pfeffer, Gerhard Dobler

p. 465

7.

Characterisation of a E2-protein mutant of Chikungunya virus and analysis of the influence of an opal stop codon by a novel infectious cDNA clone *Klaus Grywna, Pfefferle Susanne, Nadine Petersen, Christian Drosten

p. 466

8.

Application of rapid pyrosequencing assays for detection of cocirculating variants of influenza A/H3N2 viruses Marianne Wedde, Claudia Langnick, *Brunhilde Schweiger

p. 467

9.

Phylogenetic analysis of autochthonous HEV infections in Germany *Sven Schimanski, Ole Wichmann, Jürgen Wenzel, Annelie Plentz, Judith Koch, Klaus Stark, Wolfgang Jilg

p. 468

Workshop: Virus structure, assembly and egress I Chairs:

Kay Grünewald (München) Barbara Klupp (Insel Riems)

When & Where:

Friday, March 7th, 2008 10:15 – 12:00

CH – Großer Saal

1.

Cryo-Electron Microscopy Reveals that Vaccinia Virus Precursor Consists of Single Membrane and Scaffold Protein *Petr Chlanda, Alejandra Carbajal, Gareth Griffiths, Marek Cyrklaff, Jacomine Krijnse-Locker

p. 38

2.

The nuclear egress complex of human cytomegalovirus: a network of lamina-associated proteins and protein kinases *Jens Milbradt, Sabrina Auerochs, Manfred Marschall

p. 36

3.

Systemic screen for dominant negative mutants of M53 gene reveals effects on both DNA packaging and nuclear egress *Mirela Popa, Zsolt Ruzsics, Christopher Buser, Paul Walther, Ulrich H. Koszinowski

p. 34

4.

Concomitant deletion of the pUL11 and gM proteins from herpes simplex virus 1 does not severely impair virion maturation *Tobias Leege, Walter Fuchs, Martina Kopp, Harald Granzow, Barbara G. Klupp, Thomas C. Mettenleiter

p. 35

5.

Parvovirus egress from the infected cell: the role of gelsoline. *Séverine Bär, Jean Rommelaere, Jürg Nüesch

p. 42

6.

Hepatitis B virus core protein point mutations allowing capsid formation but blocking packaging of the pregenome/reverse transcription complex Alexander Pairan, *Volker Bruss

p. 40

7.

Functionally distinct subtypes of the human hepatitis B virus can be separated by their heparin binding competence *Stefan Seitz, Caroline Gähler, Anke Ostertag, Andreas Schulze, Stephan Urban

p. 46

*: Presenting author

Workshop: Antiviral therapy and resistance Chairs:

Rolf Kaiser (Köln) Holger Zimmermann (Wuppertal)

When & Where:

Friday, March 7th, 2008 10:15 – 12:00

Zool. – Großer Saal

1.

Susceptibility to antiviral drugs of influenza A viruses circulating in Germany between 1998 and 2007 *Susanne Duwe, Manuela Friedrich, Timm Harder, Brunhilde Schweiger

p. 193

2.

Cox inhibitors; a potential new way to treat HCMV disease *Joerg Schroeer, Thomas E. Shenk

p. 199

3.

Characterisation of hepatitis C virus variants resistant to the NS3/4A protease inhibitor telaprevir (VX-950) *Anna-Lena Gamer, Jennifer Schmitt, Artur Kaul, Ralf Bartenschlager

p. 194

4.

Analysis of Integrase Inhibitor (INI) resistance of HIV-1 Group M in ART-naïve and -experienced but INI-naïve patients in Germany Nadine Sichtig, *Martin Däumer, Stefan Esser, Ulf Dittmer, Eugen Schülter, Mark Oette, Stefan Reuter, Gerd Fätkenheuer, Jürgen Rockstroh, Herbert Pfister, Rolf Kaiser

p. 195

5.

Comparison of the Slopes of HIV-1 Drug Response Curves to Antiretrovirals - Addressing NRTI Drug Potency *Hauke Walter, Karin Metzner

p. 196

6.

Determination of HIV-1 coreceptor preference – Genotype vs. phenotype *Martin Obermeier, Stephan Neifer, Thomas Berg

p. 198

7.

The NF-kappaB-inhibitor SC75741 efficiently blocks influenza virus p. 197 propagation in vitro and in vivo without the tendency to induce resistant virus var *Stephan Ludwig, Christina Ehrhardt, Karoline Droebner, Andrea Rückle, Carina Banning, Eike Hrincius, Katharina Hoppe, Johann Leban, Stefan Strobl, Daniel Vitt, Oliver Planz

*: Presenting author

Workshop: Signal transduction Chairs:

Christine Ehrhardt (Münster) Eberhard Hildt (Freiburg)

When & Where:

Friday, March 7th, 2008 10:15 – 12:00

CH – Kleiner Saal

1.

p. 321 The Epstein-Barr virus oncoprotein LMP1 exploits TRADD for signaling by masking its apoptotic activity Frank Schneider, Julia Neugebauer, Janine Griese, Helmut Kutz, *Arnd Kieser

2.

EBV’s latent membrane protein 2A mimics BCR signaling *Stephanie Medele, Liudmila Matskova, Dagmar Pich, Christoph Mancao, Wolfgang Hammerschmidt

p. 325

3.

Towards the identification of the GEF activity in the Nef-Pak2 complex *Susanne Rauch, Kati Pulkkinen, Kalle Saksela, Oliver T. Fackler

p. 323

4.

Absence of apoptosis in Ebola virus-infected cells is not based on p. 324 activation of cellular survival signalling pathways *Judith Küsters, Melanie N. Grosch, Elke Mühlberger

5.

p. 322 Inhibition of HCV replication by Sorafenib/Nexavar: therapeutic potential of targeting c-Raf *Kyoshi Himmelsbach, Daniel Sauter, Thomas Baumert, Eberhard Hildt

6.

Phosphorylation of the influenza A virus PB1-F2 protein by PKC is p. 326 required for efficient virus propagation *David Mitzner, Igor Mazur, Nicole Studtrucker, Andre Eissmann, Darisuren Anhlan, Ludmilla Wixler, Josef Wissing, Lothar Jaensch, Karsten Bruns, Alok Sharma, Victor Wray, Peter Henklein, Stephan Ludwig, Ulrich Schubert

7.

Role of hypercytokinemia in the pathogenesis of H5N1 influenza virus infection in mice with altered NF-kappaB signalling pathway *Karoline Droebner, Sarah Reiling, Mathias Buettner, Oliver Planz

*: Presenting author

p. 327

Workshop: Host cell factors Chairs:

Carsten Münk (Langen) Beate Sodeik (Hannover)

When & Where:

Friday, March 7th, 2008 15:45 – 17:30

CH – Kleiner Saal

1.

Interaction of SARS-CoV Orf7a with the proapoptotic BH3-only protein bik reduces apoptosis *Claudia Diemer, Martha Schneider, Hermann Schätzl, Sabine Gilch

p. 503

2.

Human airway trypsin-like protease (HAT) cleaves the influenza virus hemagglutinin at the cell surface *Eva Böttcher, Mikhail Matrosovich, Tatyana Matrosovich, Hans-Dieter Klenk, Wolfgang Garten

p. 507

3.

Large-scale reorganization of host cell chromatin by HCMV infection *Christina Paulus, Jörg Schröer, Michael Schnitzbauer, Michael Nevels

p. 505

4.

The human exogenous gammaretrovirus XMRV preferentially uses the human Xpr1 receptor *Kristin Stieler, Carol Stocking, Marc Sitbon, Martin Aepfelbacher, Nicole Fischer

p. 504

5.

Model Structure of APOBEC3C Supports a Critical Role of Protein Dimerization for Cell-Intrinsic Antiviral Activity *Benjamin Stauch, Henning Hofmann, Martin Weisel, Klaus Cichutek, Carsten Münk, Gisbert Schneider

p. 508

6.

HIV-1 Nef Interferes with Triggered Actin Remodelling to Affect Cell Motility *Bettina Stolp, Simone Giese, Sebastian Hannemann, Oliver T. Fackler, Goulimari Polyxeni, Robert Grosse

p. 502

7.

POSH and ALIX cooperate to facilitate efficient HIV-1 budding *Jörg Votteler, Stefan Sörgel, Liane Neumann, Nicole Studtrucker, Torsten Walther, Elena Yavnilovitch, Daniel Taglicht, Yuval Reiss, Ulrich Schubert

p. 506

*: Presenting author

Workshop: Adaptive immune response and vaccination II Chairs:

Gerd Sutter (Lengen) Alexandra Trkola (Zürich)

When & Where:

Friday, March 7th, 2008 15:45 – 17:30

CH – Großer Saal

1.

p. 268 A versatile dendritic cell targeting vaccine based on coronavirus vectors induces strong and long-lasting antiviral and antitumor immunity *Divine Makia, Klara Kristin Eriksson, Luisa Cervantes-Barragán, Roland Züst, Reinhard Maier, Burkhard Ludewig, Volker Thiel

2.

Vaccine efficacy of recombinant Modified Vaccinia Virus Ankara compared to adenoviral vector expressing both respiratory syncytial virus F protein *Sarah Schwannecke, Rebekka Kohlmann, Yasemin Süzer, Tanja Jürgens, Bettina Tippler, Gerd Sutter, Klaus Überla, Thomas Grunwald

p. 269

3.

Long term immunity against novel shared MVA/smallpox T cell epitopes as identified by differential stable isotope labeling *Verena S. Meyer, Wolfgang Kastenmüller, Georg Gasteiger, Mirita Franz-Wachtel, Tobias Lamkemeyer, Hans-Georg Rammensee, Stefan Stevanovic, Dagmar Sigurdardottir, Ingo Drexler

p. 270

4.

Conditional antigen expression by replication deficient murine cytomegalovirus *Christian A. Mohr, Torsten Sacher, Zsolt Ruzsics, Ulrich H. Koszinowski

p. 260

5.

Quantization and Imaging of Cytomegalovirus Immune Evasion *Niels A. W. Lemmermann, Kerstin M. Gergely, Verena Böhm, Petra Deegen, Matthias J. Reddehase, Natascha K. Grzimek

p. 263

6.

HCMV interferes with the MHC class I peptide loading complex by inhibiting tapasin expression *Anne Halenius, Sebastian Hauka , Frank Momburg, Hartmut Hengel

p. 273

7.

Rapid reconstitution of T cell memory and remission of EBV lymphomas after therapeutic transfer of EBV-specific T cells *Andreas Moosmann, Iris Bigalke, Stefanie Tippmer, Julitta Urlacher, Wolfgang Hammerschmidt, Hans-Jochem Kolb

p. 271

*: Presenting author

Workshop: Pathogenesis and persistence I Chairs:

Jan Münch (Ulm) Norbert Tautz (Gießen)

When & Where:

Friday, March 7th, 2008 15:45 – 17:30

Zool. – Großer Saal

1.

Processing of genome 5’ termini as a strategy of negative-strand RNA viruses to avoid RIG-I-dependent interferon induction *Matthias Habjan, Ida Andersson, Jonas Klingström, Michael Schümann, Arnold Martin, Petra Zimmermann, Valentina Wagner, Andreas Pichlmair, Urs Schneider, Elke Mühlberger, Ali Mirazimi, Friedemann Weber

p. 391

2.

Correlation between cofactor dependency and pestiviral persistence *Deborah Basso, Heinz-Jürgen Thiel, Norbert Tautz

p. 402

3.

Identification and characterisation of MCMV encoded miRNAs in vitro and in vivo *Lars Dölken, Zsolt Ruzsics, Jonathan Perot, Soutschek Jürgen, Matthias John, Sébastien Pfeffer, Ulrich H. Koszinowski

p. 392

4.

The murine cytomegalovirus m38.5 protein inhibits Bax-mediated cell death *Uwe Schumacher, Igor Jurak, Hrvoje Simic, Sebastian Voigt, Wolfram Brune

p. 394

5.

In vivo conditional depletion of latent murine Cytomegalovirus *Sabine Scheller, Christian O. Simon, Jürgen Podlech, Petra Deegen, Natascha K. A. Grzimek

p. 396

6.

The Epstein-Barr virus-encoded protein BDLF2 is influencing the actin cytoskeleton resulting in enlarged cell surface and increased motility Jens Bernhard Lösing, Klaus Ritter, *Michael Kleines

p. 399

7.

Interferon-gamma influences the pathogenesis after H5N1 influenza p. 401 A virus infection in mice *Emanuel Haasbach, Sarah Reiling, Mathias Buettner, Oliver Planz

*: Presenting author

Workshop: Virus structure, assembly and egress II Chairs:

Kay Grünewald (München) Barbara Klupp (Insel Riems)

When & Where:

Saturday, March 8th, 2008 09:15 – 11:00

CH – Großer Saal

1.

The role of actin in polarized release of measles virus *Erik Dietzel, Nicole Runkler, Andrea Maisner

p. 39

2.

Studying association of influenza virus HA with membrane-rafts using fluorescence-resonance-energy transfer (FRET) *Stephanie Engel, Nils Krebs, Silvia Scolari, Claudia Tielesch, Andreas Herrmann, Michael Veit

p. 37

3.

Late domains of the Marburg virus nucleoprotein and their role in Marburg virus-like particle release. *Olga Dolnik, Florian Zielecki, Lea Stevermann, Larissa Kolesnikova, Stephan Becker

p. 44

4.

Dissecting the Role of Cellular Factors in Retroviral Budding using Heterodimerizer-mediated Virus-Host Protein Interaction *Stefanie Jäger, Ivonne Morales, Anja Habermann, Hans-Georg Kräusslich

p. 47

5.

HIV-1 transframe protein p6*, a spacer protein dispensable for viral in vitro replication *Andreas Leiherer, Christine Ludwig, Ralf Wagner

p. 41

6.

Structural and stoichiometrical Studies on Ribonucleoprotein Complex Components of Borna Disease Virus Ina Kraus, Guy Schoehn, Marc Jamin, Wolfgang Garten, Winfried Weissenhorn, *Miriam Hock

p. 45

7.

Essential role of domain III of non-structural protein 5A for Hepatitis C virus infectious particle assembly *Margarita Zayas, Nicole Appel, Sven Miller, Jacomine Krijnse-Locker, Torsten Schaller, Peter Friebe, Stephanie Kallis, Ulrike Engel, Ralf Bartenschlager

p. 43

*: Presenting author

Workshop: Pathogenesis and persistence II Chairs:

Jan Münch (Ulm) Norbert Tautz (Gießen)

When & Where:

Saturday, March 8th, 2008 09:15-11:00

Zool. – Großer Saal

1.

The role of annexin A2 in the hepatitis C virus life cycle and potential implications for pathogenesis *Perdita Backes, Simon Reiss, Doris Quinkert, Thomas Longerich, Volker Gerke, Ralf Bartenschlager, Volker Lohmann

p. 393

2.

Limitation of hepatitis C virus (HCV) escape from a dominant HLAB27 restricted CD8+ T-cell epitope by replication fitness *Eva Dazert, Christoph Neumann-Haefelin, Nicole Appel, Susan McKiernan, Dermot Kelleher, Robert Thimme, Ralf Bartenschlager

p. 398

3.

Mouse Hepatitis Virus liver pathology is dependent on ADP-ribose 1''-phosphatase, a viral function conserved in the alpha-like supergroup Klara Kristin Eriksson, Luisa Cervantes-Barragán, Burkhard Ludewig, *Volker Thiel

p. 400

4.

Exploring new mechanisms of antiviral activity of APOBEC3C *Henning Hofmann, Klaus Cichutek, Carsten Münk

p. 403

5.

Evaluation of a HIV integrase inhibitor in the transgenic rat model of HIV infection *Christine Goffinet, Ina Allespach, Lena Oberbremer, Pamela Golden, Scott Foster, Johns Brian, Weatherhead Jason, Novick Steven, Chiswell Karen, Garvey Edward, Keppler Oliver T.

p. 404

6.

Inhibition of Transferrin-Receptor Uptake by SIV and HIV Nefalleles *Swantje Gundlach, Carina Banning, Alicja Iwanski, Frank Kirchhoff, Michael Schindler

p. 395

7.

p. 397 Determinants of the HIV pathogenesis factor Nef for efficient virus replication and CD4 T lymphocyte depletion in ex vivo human tonsil histocultures *Homann Stefanie, Tibroni Nadine, Baumann Ingo, Sertel Serkan, Keppler Oliver T. , Fackler Oliver T.

*: Presenting author

Workshop: Viral RNA genome replication Chairs:

Elke Mühlberger (Marburg) Volker Thiel (St. Gallen)

When & Where:

Saturday, March 8th, 2008 09:15 – 11:00

CH – Kleiner Saal

p. 125

1.

RNA replication of Tomato bushy stunt virus in a plant-derived in vitro system *Torsten Gursinsky, Beate Schulz, Sven-Erik Behrens

2.

p. 126 Genome Organization and Reverse Genetic Analysis of a Type I Feline Coronavirus *Gergely Tekes, Regina Hofmann-Lehmann, Iris Stallkamp, Volker Thiel, Heinz-Jürgen Thiel

3.

Structural and functional characterization of the chymotrypsin-like protease of the human pathogenic sapovirus Ivonne Robel, Julia Gebhardt, Jeroen Mesters, Alexander Gorbalenya, Bruno Coutard, Bruno Canard, Rolf Hilgenfeld, *Jacques Rohayem

p. 121

4.

Localization determines function: N-terminally truncated NS5A fragments accumulate in the nucleus and impair HCV replication *Daniel Sauter, Kyoshi Himmelsbach, Malte Kriegs, Eberhard Hildt

p. 122

5.

Novel function of the influenza A virus PB1-F2 protein – Regulation of viral polymerase activity by interaction with the PB1 polymerase subunit Igor Mazur, Darisuren Anhlan, David Mitzner, Ludmilla Wixler, Ulrich Schubert, *Stephan Ludwig

p. 124

6.

Characterization of the Ebola virus VP30 – NP interaction *Nadine Biedenkopf, Bettina Hartlieb, Stephan Becker

p. 123

7.

Foamy virus protein Bet alters biochemical properties of APOBEC3 proteins *Mario Perkovic, Benjamin Stauch, Henning Hofmann, Stanislaw Schmidt, Ferdinand Kopietz, Gisbert Schneider, Martin Löchelt, Klaus Cichutek, Carsten Münk

p. 127

*: Presenting author